X-gluc 5-溴-4氯-3-吲哚葡萄糖苷

X-gluc

Description: X-gluc描述

In order to identify transformed cells or plants that have been growing on a selective medium, it is necessary to have an easily assayable reporter gene.

(X-glu, X-glcA) 5-Bromo-4-chloro-3-indoxyl-beta-D-glucuronide cyclohexylammonium salt

Sold as  1 g units

MOLECULAR BIOLOGY GRADE:C₁₄ H₁₃ Br Cl N O₇? C₆ H₁₃ N

M.W.:521.79

Purity: greater than 99 (HPLC)

Thin Layer Chromatogram: single spot

Biological Activity: indigo-blue assay

Elemental Analysis: Agrees with theoretical ＆#118alues

CAS RN: [18656-96-7]

GUS Staining of Pollen：操作步骤

1. Add 200μl of acetone, vortex briefly.

2. Remove acetone and pipette into flat-bottom 96-well plate (polystyrene)

3. Centrifuge plate at 3000 x g for 3-5 minutes.

4. Invert plate carefully and blot dry with paper towels

5. Add 40μl of X-Gluc stain solution to each well (multi-pipettor) and incubate at

37°C overnight.

X-Gluc Solution:溶液配制

1 20 mg/ml X-Gluc concentration. e.g. For a 1.5 ml tube (microfuge tube)

dissolve 30 mg ( .03 g ) of X-Gluc in 1.5 ml DMSO (dimethyl sulfoxide).

2 Cover with foil.

3 Vortex well.

4 Keep at -20°C .

X-Gluc stain solution：解决方法

*Note: 4ml of stain solution are necessary to stain 1 96-well plate.

References using GBT X-Gluc：相关论文

1. Ourmanov, I. et al. (2009). Improved Survival in Rhesus Macaques Immunized with Modified Vaccinia Virus. Journal of Virology, 83(11), 5388-5400.

2. Coque, L. et al. (2008). Transcription of ENOD 8 in Medicago truncatula Nodules Directs ENOD8 Esterase to Developing and Mature Symbiosomes. Molecular Plant-Microbe Interactions, 21(4), 404-410.

3. Kim, H., & Woloshuk, C.P. (2008). Role of AREA, a regulator of nitrogen metabolism, during colonization of maize kernels and fumonisin biosynthesis in Fusarium verticillioides. Fungal Genetics and Biology, 45 (6), 947-953.

4. Scarpeci, T.E. (2008). Generation of superoxide anion in chloroplasts of Arabidopsis thaliana during active photosynthesis: a focus on rapidly induced genes . Plant Molecular Biology, 66(4), 361-378.

5. Geddie, M.L., & Matsumura, I. (2007). Antibody-Induced Oligomerization and Activation of an Engineered Reporter Enzyme. Journal of Molecular Biology, 369 (4), 1052-1059.

6. Ko et al. (2004). In Vitro Cellular and Developmental Biology- Plant, 40 (6). 552-558.

7. Curtis et al. (2000). Annals of Botany, 86. 63-71.

8. Ingram et al. (2000).Plant Cell, Tissue and Organ Culture, 57. 207-210.

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