翌圣明星CP--转染试剂与PCR产品又登《Cell》期刊

2022年3月17日，西湖大学生命科学学院陶亮团队在《Cell》期刊（IF：41.582）在线发表了题为《TFPI is a colonic crypt receptor for TcdB from hypervirulent clade 2 C. difficile》的研究论文。该研究对于揭示和理解艰难梭菌感染的致病机制，梭菌毒素的进化和工作方式有着重要科学意义，并为艰难梭菌感染的预防和治疗提供了重要的理论依据。

 

  图片来源：论文截图     团队在该研究中选择了翌圣的转染试剂与PCR产品用于相关基因的研究：     目前转染试剂系列与PCR系列的产品已经荣登《Nature》《Cell》等多个顶-级期刊，获得科研大牛们认可！
  以下仅展示部分论文：

转染试剂系列：

 

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[1] Liu R, Yang J, et al. Optogenetic control of RNA function and metabolism using engineered light-switchable RNA-binding proteins. Nat Biotechnol. 2022 Jan 3. (IF 55)华东理工大学生物工程学院杨弋团队

[2] Zhou J, Chen P, et al. Cas12a variants designed for lower genome-wide off-target effect through stringent PAM recognition. Mol Ther. 2022 Jan 5.(IF 11.454)武汉大学生命科学学院殷雷团队

[3] Chen S, Cao X, et al. circVAMP3 Drives CAPRIN1 Phase Separation and Inhibits Hepatocellular Carcinoma by Suppressing c-Myc Translation. Adv Sci (Weinh). 2022 Jan 24.(IF 16.808)中国科学院北京生命科学研究院赵方庆团队

[4] Gu C, Wang Y, et al. AHSA1 is a promising therapeutic target for cellular proliferation and proteasome inhibitor resistance in multiple myeloma. J Exp Clin Cancer Res. 2022 Jan 6.(IF 11.161)南京中医药大学杨烨顾春艳团队

[5] Zhang Y, Yu X, et al. Splicing factor arginine/serine-rich 8 promotes multiple myeloma malignancy and bone lesion through alternative splicing of CACYBP and exosome-based cellular communication. Clin Transl Med. 2022 Feb.(IF 11.492)南京中医药大学杨烨顾春艳团队

[6] Qin J, Cai Y, et al. Molecular mechanism of agonism and inverse agonism in ghrelin receptor. Nat Commun. 2022 Jan 13.(IF 14.9)四川大学生物治疗国家重点实验室邵振华团队

[7] Tang X, Deng Z, et al. A novel protein encoded by circHNRNPU promotes multiple myeloma progression by regulating the bone marrow microenvironment and alternative splicing. J Exp Clin Cancer Res. 2022 Mar 8.(IF 11.161)南京中医药大学杨烨顾春艳团队

[8] Xie F, Su P, et al. Engineering Extracellular Vesicles Enriched with Palmitoylated ACE2 as COVID-19 Therapy. Adv Mater. 2021 Oct 19. (IF 30.849)苏州大学生物医学研究院周芳芳团队和浙江大学生命科学研究院张龙团队

[9] Liang Y, Lu Q, et al. Reactivation of tumour suppressor in breast cancer by enhancer switching through NamiRNA network. Nucleic Acids Res. 2021 Sep 7.(IF 16.9)复旦大学生物医学研究院于文强团队

[10] Fan Y, Wang J, et al. CircNR3C2 promotes HRD1-mediated tumor-suppressive effect via sponging miR-513a-3p in triple-negative breast cancer. Mol Cancer. 2021 Feb 2.(IF 27.403)南京医科大学附属逸夫医院苏东明团队

[11] Dai L, Dai Y, et al. Structural insight into BRCA1-BARD1 complex recruitment to damaged chromatin. Mol Cell. 2021 Jul 1.(IF 17.97)浙江大学生命科学研究院黄俊团队和中科院生物物理所周政团队

[12] Zhang K, Wang A, et al. UBQLN2-HSP70 axis reduces poly-Gly-Ala aggregates and alleviates behavioral defects in the C9ORF72 animal model. Neuron. 2021 Jun 16.(IF 17.17)中国科学院生物与化学交叉研究中心王文元团队

[13] Li T, Chen X, et al. A synthetic BRET-based optogenetic device for pulsatile transgene expression enabling glucose homeostasis in mice. Nat Commun. 2021 Jan 27.(IF 14.92)华东理工大学生物工程学院杨弋团队

[14] Pan Y, He X, et al. Neuronal activity recruits the CRTC1/CREB axis to drive transcription-dependent autophagy for maintaining late-phase LTD. Cell Rep. 2021 Jul 20.(IF 9.420)浙江大学脑科学与脑医学学院马欢团队

[15] Liu H, Xing R, et al. G-protein-coupled receptor GPR17 inhibits glioma development by increasing polycomb repressive complex 1-mediated ROS production. Cell Death Dis. 2021 Jun 12.(IF 8.463)厦门大学生命科学学院陈颖团队

[16] Yan F, Huang C, et al. Threonine ADP-Ribosylation of Ubiquitin by a Bacterial Effector Family Blocks Host Ubiquitination. Mol Cell. 2020 May 21.(IF 17.97)浙江大学生命科学研究院朱永群团队

[17] Luo Q, Wu X, et al. TRIM32/USP11 Balances ARID1A Stability and the Oncogenic/Tumor-Suppressive Status of Squamous Cell Carcinoma. Cell Rep. 2020 Jan 7.(IF 9.42)中国医学科学院分子肿瘤学国家重点实验室刘芝华团队

[18] Sun X, Peng X, et al. ADNP promotes neural differentiation by modulating Wnt/β-catenin signaling. Nat Commun. 2020 Jun 12.(IF 14.911)中国科学院水生生物研究所孙玉华团队

[19] Yang X, Wang H, et al. Rewiring ERBB3 and ERK signaling confers resistance to FGFR1 inhibition in gastrointestinal cancer harbored an ERBB3-E928G mutation. Protein Cell. 2020 Dec.(IF 14.872)浙江大学医学院/转化医学研究院闵军霞团队

[20] Zou Y, Wang A, et al. Analysis of redox landscapes and dynamics in living cells and in vivo using genetically encoded fluorescent sensors. Nat Protoc. 2018 Oct.(IF 13.490)华东理工大学生物工程学院杨弋、赵玉政团队

[21] Zhang K, Zhao X, et al. Enhanced Therapeutic Effects of Mesenchymal Stem Cell-Derived Exosomes with an Injectable Hydrogel for Hindlimb Ischemia Treatment. ACS Appl Mater Interfaces. 2018 Sep 12.(IF 8.09)南开大学医学院李宗金团队

[22] Hao H, Hu S, et al. Loss of Endothelial CXCR7 Impairs Vascular Homeostasis and Cardiac Remodeling After Myocardial Infarction: Implications for Cardiovascular Drug Discovery. Circulation. 2017 Mar 28.(IF 29.69)中国医学科学院/北京协和医学院阜外医院王淼团队

    PCR系列：  

 

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[1] Yu, Q., Liu, S., Yu, L. et al. RNA demethylation increases the yield and biomass of rice and potato plants in field trials. Nat Biotechnol (2021). （IF 54.908）

[2] Dong W, Zhu Y, Chang H, et al. An SHR–SCR module specifies legume cortical cell fate to enable nodulation[J]. Nature, 2021, 589(7843): 586-590. （IF43）

[3] Bi X, Wang K, Yang L.et al. Tracing the genetic footprints of vertebrate landing in non-teleost ray-finned fishes. Cell. (2021).  (IF 38.637)

[4] Liu S , Hua Y , Wang J , et al. RNA polymerase III is required for the repair of DNA double-strand breaks by homologous recombination[J]. Cell. (2021). (IF 38.637)

[5] Liu C , Li X. et.al. Structure and Degradation of Circular RNAs Regulate PKR Activation in Innate Immunity. Cell. (2019).  (IF 36.216)

[6] Lu, XY.,Shi, XJ., Hu, A.et al.Feeding induces cholesterol biosynthesis via the mTORC1–USP20–HMGCR axis.Nature(2020). （IF 43）

    科研路上高分paper发不停，小翌助您过关斩将，每天都出好数据。

 

 

表1. 翌圣生物转染试剂产品目录

名称	货号	规格	价格（元）
Hieff TransTM Liposomal Transfection Reagent脂质体核酸转染试剂	40802ES01	100 μL	168
	40802ES02	0.5 mL	738
	40802ES03	1.0 mL	1308
	40802ES08	5×1mL	4878
Calcium Phosphate Cell Transfection Kit 磷酸钙法细胞转染试剂	40803ES70	200 T	625
Polybrene (hexadimethrine bromide) 聚凝胺（10 mg/ml）	40804ES76	500 μL	180
	40804ES86	5×500 μL	500
Hieff TransTM Suspension Cell-Free Liposomal Transfection Reagent 悬浮细胞专用脂质体核酸转染试剂	40805ES01	100 μL	228
	40805ES02	0.5 mL	948
	40805ES03	1.0 mL	1678
	40805ES08	5×1 mL	5268
Hieff TransTM in vitro siRNA/miRNA Transfection Reagent siRNA/miRNA体外转染试剂	40806ES01	0.1 mL	372
	40806ES02	0.5 mL	1472
	40806ES03	1.0 mL	2572
Polyethylenimine Linear(PEI) MW25000 线性PEI转染试剂MW25000	40815ES03	1 g	1855
	40815ES08	5×1 g	7255
Polyethylenimine Linear(PEI) MW40000（rapid lysis）线性PEI转染试剂（速溶型）MW40000	40816ES02	100 mg	655
	40816ES03	1 g	1855
	40816ES08	5×1 g	7255

 

点击产品名称即可查看详情   表2. 翌圣生物RT-qPCR产品目录

 

方法	分类	产品名称	货号
qPCR染料法	高灵敏通用性qPCR预混液（染料法）	Hieff UNICON® Universal Blue qPCR SYBR® Master Mix（男神版）	11184ES
	高特异型qPCR预混液（染料法）	Hieff UNICON® Power qPCR SYBR® Green Master Mix  (No Rox)	11195ES
		Hieff UNICON® Power qPCR SYBR® Green Master Mix(Low Rox)	11196ES
		Hieff UNICON® Power qPCR SYBR® Green Master Mix(High Rox)	11197ES
	高灵敏型qPCR预混液（染料法）	Hieff UNICON® qPCR SYBR® Green Master Mix (No Rox)	11198ES
		Hieff UNICON® qPCR SYBR® Green Master Mix (Low Rox)	11199ES
		Hieff UNICON® qPCR SYBR® Green Master Mix (High Rox)	11200ES
	普通型qPCR预混液（染料法）	Hieff® qPCR SYBR® Green Master Mix (No Rox )	11201ES
		Hieff® qPCR SYBR® Green Master Mix (Low Rox )	11202ES
		Hieff® qPCR SYBR® Green Master Mix (High Rox )	11203ES
	miRNA qPCR加A法	Hieff® miRNA Universal  qPCR SYBR Master Mix（加A法）	11171ES
	miRNA qPCR茎环法	Hieff® miRNA Universal  qPCR SYBR Master Mix（茎环法）	11170ES
	RNA一步法RT-qPCR（染料法）	Hifair®  III One Step RT-qPCR SYBR Green Kit	11143ES
qPCR探针法	单重、普通型qPCR预混液（探针法）	Hieff Unicon® qPCR TaqMan Probe Master Mix	11205ES
	多重、高灵敏型qPCR预混液（探针法）	Universal TaqMan multiplex qPCR master mix	11211ES
	RNA一步法RT-qPCR（探针法）	Hifair®  III One Step RT-qPCR Probe Kit	11145ES

 

点击产品名称即可查看详情