Leukemia Inhibitory Factor, recombinant rat 重组大鼠白血病抑制因子LIF3010/LIF3005

• Leukemia Inhibitory Factor, recombinant rat

   现货供应
   
  Description: Leukemia Inhibitory Factor, recombinant rat

   

  Trade Name: Chemicon (Millipore)

   

  Product Overview: Rat LIF (rtLIF) is a 19.9 kDa protein containing 183 amino acid residues that exhibits 91% amino acid sequence identity with murine LIF (Takahama etal 1998). Analysis of the secondary structure of rat and murine LIF indicates a difference in the two dimensional structure of both proteins. Studies have shown that rtLIF is more effective in maintaining the undifferentiated phenotype of rat ES cells than similar concentrations of murine LIF (Takahama, et al 1998).
  
  Manufactured by CHEMICON International, Inc. Rat LIF is not available for resale.
  
  For research use only; not for use as a diagnostic.

   

  Background Information: Leukemia Inhibitory Factor (LIF) is a lymphoid factor which promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. LIF has a number of other activities including cholinergic neuron differentiation, control of stem cell pluripotency, bone and fat metabolism, mitogenesis of certain factor dependent cell lines and promotion of megakaryocyte production in vivo.

   

  Key Applications:

  • Stem Cell Culture
  • Cell Culture

   

  Application Notes: RECOMMENDED QC PROTOCOL
  
  M1 Bioassay
  
  1.The M1 bioassay is performed using in vitro semi-solid agar cultures, which contain approximately 100 cells in 1 mL volumes of DME containing 20 % FCS in 0.3% agar.
  
  2.Add 100 μL of sample or rtLIF (10(e4) units/mL in 5% FCS in isotonic saline) in two-fold serial dilutions in duplicate to 35 mm petri dishes.
  
  3.Add 100 μL of 5% FCS in isotonic saline to two control slides.
  
  4.Incubate at 37°C in fully humidified atmosphere of 10% CO2 in air for 7 days.
  
  5.Score the number of colonies that show differentiation (note: 50 units is defined as the amount of activity which results in 50% of the colonies being differentiated).
  
  Visit www.esgro-lif.com for additional information

   

  Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

   

  Species: Rat

   

  Stem Cell Type:

  • Mouse Embryonic Stem Cells
  • Neural Stem Cells
  • Hematopoietic Stem Cells
  • Mesenchymal Stem Cells
  • Induced Pluripotent Stem Cells

   

  Stem Cell Workflow Stage:

  • Maintenance & Expansion
  • Differentiation
  • General Cell Culture

   

  Source: rtLIF is expressed in E. coli as a fusion protein with GST using the pGEX expression system, cleaved from GST moiety with thrombin and purified by ion-exchange chromatography.

   

  Presentation: Liquid in 50mM sodium phosphate/1mM DTT / 10% glycerol / 250mM NaCl, pH 7.4 and 0.02% Tween 20. No preservativies added.

   

  Storage Conditions: Rat LIF is shipped on dry ice. Maintain at -20°C until expiration date. Further dilutions should be made into buffer or medium to which protein (e.g., 1% BSA) or Tween 20 has been added and stored at -20°C. Freeze thawing is not recommended.

   

  Specific Activity: The activity of rat LIF is determined by the ability to induce differentiation of M1 myeloid leukemic cells. The minimum detectable concentration of rat LIF in this assay is 0.5 ng/mL. The specific activity is >=1 x 10(e8) units/mg, where 50 units is defined as the amount of rat LIF required to induce differentiation in 50% of the M1 colonies in 1 mL agar cultures.

   

  Cell Culture Reagent Type: Cytokines & Growth Factors

   

  Gene Symbol: NM_022196

   

  Protein or Enzyme Type: Cytokines & Growth Factors

   

  Alternate Names: LIF

   

  Concentration: 10 μg/mL

   

  Purity: Greater than 85% by analytical HPLC and SDS-PAGE. Endotoxin level is less than 0.1 ng per μg of LIF. Tested negative in both aseptic and mycoplasma tests. 

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