FuGENE® HD Transfection Reagent

FuGENE^® HD Transfection Reagent

Application

FuGENE^® HD Transfection Reagent is a next-generation transfection reagent, free of animal-derived components. It is designed to transfect a wide range of eukaryotic cells including insect cells and many cell lines not transfected well by other reagents (e.g .,  MCF-7, RAW 264.7, PC-3, HeLa, MA-10, HepG2, SH-SY5Y, A7r5, STO, SCC-61, STSAR-90, SQ20B, T98).

Note: For the most up-to-date list of cell types that have been successfully transfected with FuGENE^® HD Transfection Reagent, visit our Transfection Special Interest Site at www.powerful-transfection.com and view our Transfection Reference Database. This site also contains much more information about the use of this reagent.

For life science research only.

Product Description

Composition: unique and proprietary blend of many components,  in 80% ethanol.

Storage:  stable at + 2°C to + 8°C .

Stability: 2 years, starting from date of manufacture.

Contents:

Solution in 80% ethanol, filter-sterilized (0.1 µm filter), packaged in transparent glass vials.

Quality:

Lot-specific analysis: 

Each lot of FuGENE^®HD Transfection Reagent is carefully checked, using ISO DIN 9001-certified procedures, to assure product is performing according to specifications and is consistent from lot to lot.

Functional analysis:

Cos 7 cells are transfected in 96 well plates with a reporter gene vector DNA, using 0.3 µl FuGENE^® HD and 0.1 µg DNA or 0.25 µl FuGENE^® HD and 0.1 µg DNA. Reporter gene activity is monitored via a colorimetric test against a reference lot. Activity must be ≥ 75% compared to a reference lot in at least one of the tested amounts, listed above.

Cytotoxicity analysis:

Cell growth is assessed using the Cell Proliferation Reagent WST-1assay  which is an indirect measure of cytotoxicity. The remainder of cells from functional analysis is tested for viability.

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