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Annexin-V-Biotin 细胞凋亡及细胞死亡

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Annexin-V-Biotin 细胞凋亡及细胞死亡

1489 人阅读发布时间：2010-03-07 11:29

Annexin-V-Biotin，现货供应！Roche经典产品！

Application

The analysis of phosphatidylserine on the outer leaflet of apoptotic cell membranes is performed using biotin-labeled Annexin-V. Additionally, staining with propidium iodide (PI) in FACS analysis or with a cell surface marker can be used for differentiation from necrotic cells. Annexin-V-Biotin can be used in immunohistochemistry with a secondary detection system (e.g., Streptavidin-POD conjugate).

Product Description

Detection: Annexin-V-Biotin can be used in immunohistochemistry with a secondary detection system (e.g., Streptavidin-POD conjugate).
Sample material: Cell lines and freshly isolated cells
Specificity: Annexin-V binds in a calcium-dependent manner to negatively charged phospholipid surfaces, and shows high affinity for phosphatidylserine.

Background Information

In the early stages of apoptosis there are changes occurring on the cell surface, specifically translocation of phosphatidylserine (PS) from the interior side of the plasma membrane to the outer leaflet. This secretion of phosphatidylserine is the ideal basis for Annexin-V, which binds to PS with high affinity. Annexin-V is a Ca²⁺-dependent phospholipid-binding protein with a high affinity for PS. Since necrotic cells also expose PS as a result of lost membrane integrity, apoptotic cells must be differentiated from these necrotic cells. The simultaneous application of a DNA stain, which is used for dye-exclusion testing, allows the discrimination of necrotic cells from the Annexin-V positively stained cell cluster.

Contents
500 µl, ready-to-use solution, for 250 tests

Typical Experiment

Induce apoptosis.
Wash the cells in PBS.
Incubate cells with Annexin-V-Biotin in a Hepes buffer that contains PI or other labeling reagent for cell surfaces.
Analyze samples under a microscope or on a flow cytometer.

订购指南：以下产品部分现货供应

检测方法	产品名称	产品货号	规格	价格￥
FACS Fluorescence or light microscopies	Annexin-V-FLUOS	11828681001	250T	5023
	Annexin-V-Alexa 568	03703126001	250T	5443
	Annexin-V-Biotin	11828690001	250T	5023
	Annexin-V-FLUOS Staining Kit	11858777001	50T	1932
	Annexin-V-FLUOS Staining Kit	11988549001	250T	6233
ELISA	Caspase 3 Activity Assay	12012952001	96T	6578
ELISA	Cell Death Detection ELISA^PLUS	11774425001	96T	3814
TUNEL	In Situ Cell Death Detection Kit Fluorescein	11684795910	50T	4662
	In Situ Cell Death Detection Kit AP	11684817910	50T	5267
	In Situ Cell Death Detection Kit POD	11684817910	50T	5267
	In Situ Cell Death Detection Kit TMR red	12156792910	50T	3931

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