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Annexin-V-FLUOS Staining Kit 细胞凋亡

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Annexin-V-FLUOS Staining Kit 细胞凋亡

2057 人阅读发布时间：2011-10-17 20:24

Annexin-V-FLUOS Staining Kit，现货供应！Roche经典产品！

Application

Labeled Annexin-V, with its high affinity for phosphatidylserine (PS), is a sensitive probe for PS exposed on the outer layer of apoptotic cells. Since necrotic cells also expose PS as a result of lost membrane integrity, propidium iodide is utilized as a DNA stain to distinguish necrotic cells from Annexin-V-labeled cell clusters. Other secondary labeling is possible, such as membrane-surface staining with phycoerythrin- or TRITC-labeled monoclonal antibodies for further cellular characterization.

Product Description

Detection: Annexin-V-FLUOS can be directly detected in FACS analysis and immunochemistry without a secondary detection system.
Sample material: Cell lines and freshly isolated cells.
Specificity: Annexin-V-FLUOS binds in a Ca²⁺-dependent manner to negatively charged phospholipid surfaces, and shows high specificity for phosphatidylserine. Therefore, it stains apoptotic and necrotic cells. Propidium iodide stains only the DNA of leaky necrotic cells and allows for a distinction between apoptotic and necrotic cells.

Background Information

In the early stages of apoptosis, changes occur at the cell surface. One of these plasma membrane alterations is the translocation of phosphatidylserine (PS) from the inner side of the plasma membrane to the outer layer, exposing PS at the surface of the cell. Macrophages specifically recognize PS exposed on the cell surface of lymphocytes during the development of apoptosis. The recognition and phagocytosis of apoptotic cells and bodies protects organisms from exposure to cellular compounds that lead to inflammation, which usually accompanies necrosis. Annexin V is a Ca²⁺-dependent phospholipid-binding protein with a high affinity for PS, making labeled annexin an excellent detection agent.

订购指南：以下产品部分现货供应

检测方法	产品名称	产品货号	规格	价格￥
FACS Fluorescence or light microscopies	Annexin-V-FLUOS	11828681001	250T	5023
	Annexin-V-Alexa 568	03703126001	250T	5443
	Annexin-V-Biotin	11828690001	250T	5023
	Annexin-V-FLUOS Staining Kit	11858777001	50T	1932
	Annexin-V-FLUOS Staining Kit	11988549001	250T	6233
ELISA	Caspase 3 Activity Assay	12012952001	96T	6578
ELISA	Cell Death Detection ELISA^PLUS	11774425001	96T	3814
TUNEL	In Situ Cell Death Detection Kit Fluorescein	11684795910	50T	4662
	In Situ Cell Death Detection Kit AP	11684817910	50T	5267
	In Situ Cell Death Detection Kit POD	11684817910	50T	5267
	In Situ Cell Death Detection Kit TMR red	12156792910	50T	3931

免费咨询/订购电话：4006-111-883

Cell Sciences中国区域代理

Annexin-V-Biotin 细胞凋亡及细胞死亡

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